Abstract
Kinetic studies and product characterization of the acceptor reaction of a highly purified dextransucrase isolated from cultures of Leuconostoc mesenteroides suggested that the introduction of increasing amounts of maltose in the medium of reaction produced an increase of the kinetic constants, K′ m and V max, of the enzyme for sucrose. A Box-Hunter mathematical optimization concerning the products of the reaction with maltose showed that the sucrose-to-maltose ratio is of prime influence upon the yield of the oligosaccharides produced and that their weight-average molecular weight ( M w) is a linear function of that ratio. In view of directly synthesizing controlled-molecular-weight dextrans, various populations of oligosaccharides were produced and used as glucosyl acceptors in a second-step reaction with sucrose. The molecular weight and polydispersity of the second-step products were related to the sucrose-to-acceptor ratio and to the characteristics of the oligosaccharide acceptor.
Published Version
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