Abstract
Abiotic stresses such as photoperiod, aeration, CO2 concentration, pH, temperature, and salinity can affect and salinity the physiological processes of microalgae. One of the physiological processes that occur is a change in the lipid biosynthetic pathway towards the formation and accumulation of neutral lipids. Neutral lipid accumulation in the form of TAGs. TAGs as one of the defense mechanisms. The regulation of fatty acid biosynthesis is initiated by the ACC1 and ACC2 genes. This study aims to determine the expression of ACC1 and ACC2 genes under conditions of aeration stress and photoperiod simultaneously in microalgae Skeletonema costatum. The method used was the analysis of gene expression in the sample of S. costatum RNA extract using qRT-PCR. Based on the results of the study, it was found that the ACC1 gene was expressed and upregulated in the A2F2 treatment (24-hour aeration and 24:0 photoperiod) with a CT value of 36.85 while the ACC2 gene was expressed and upregulated in the A1F1 treatment (12-hour aeration and 12:12 photoperiod). with the CT value of 36,874.
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