Acaricide Resistance in Northern Fowl Mite (Ornithonyssus sylviarum) Populations on Caged Layer Operations in Southern California

Abstract

Southern California caged layer operations were visited over 3 yr. Northern fowl mites from 26 field populations were tested for acaricide resistance using a capillary pipette and glass dish bioassay. One was a susceptible field population with no pesticide exposure for over 30 yr (reference site for resistance ratio calculation). Technical and commercial formulations of malathion, carbaryl (Sevin), permethrin, and a commercial formulation of tetrachlorvinphos/dichlorvos (Ravap) were tested. Malathion did not have high activity for mites relative to other materials, but resistance to both technical and commercial formulations was low (< 5×). Resistance to other materials was moderate to extreme. Frequency of carbaryl resistance (> 10×) was higher with the commercial (88%) than the technical material (41%); 19% of the populations had resistance > 100x to commercial carbaryl. Frequency of Ravap resistance (> 10×) was 68%; 8% of populations had resistance > 100x. Frequency of permethrin resistance (> 10×) was 72% for the technical material and 88% for the commercial formulation. Extreme permethrin resistance (> 1,000×) was observed in 56 and 50% of mite populations assayed using the technical and commercial formulations, respectively. Among sites, resistance to permethrin was uncorrelated with resistance to other chemicals, suggesting a different resistance mechanism. Resistance to carbaryl and Ravap was highly correlated [r = 0.76 at the LC50 level (concentrations estimated to be lethal to 50% of the test population) and r = 0.99 at the LC95 level], suggesting a common resistance mechanism. Producers currently depend completely on pesticides to control mite infestations. Mite resistance to registered materials emphasizes the need for integrated control measures.