Abstract
Two spotted spider mite Tetranychus urticae Koch is an economically serious pest posing threat to major vegetable crops. Roving survey in and around Coimbatore region revealed that farmers do not target mites with acaricides instead they use higher dose of insecticides at frequent intervals which results in development of resistance. The bioassay results revealed that fenpropathrin (2.07 to 6.86-folds) and fenazaquin (2.74 to 7.13-folds) exhibit higher susceptibility, whereas diafenthiuron (5.35 to 12.25-folds) revealed a low to moderate level of resistance. The propargite (43.80 to 60.63-folds) and chlorfenapyr (61.01 to 75.10-folds) exhibited high resistance, followed by spiromesifen (222.28 to 300.26-folds) and buprofezin (382.60 to 417.87-folds), with extremely high level of resistance. The higher specific activity of GST (4.54-folds), MFO (10.06-folds) and CarE (15.06-folds) in Puthupalayam population suggested the role of biochemical resistance. A significant positive correlation was observed between diafenthiuron and CarE activity (r = 0.981*), fenpropathrin and MFO activity (r = 0.964*).
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