Abstract
Recognizable changes in the structure of Acanthamoeba castellanii (Neff strain) 1 1 ]The Neff strain is considered synonymous with A. castellanii ( Adam, 1964). during antiserum immobilization are described and documented with phase-contrast photomicrographs. Antisera against A. castellanii used in this test failed to react with cultures of A. polyphaga, A. astronyxis, and A. palestinensis. Direct microscopic observations of the sequential changes in morphologic features (i.e., contraction, vacuole formation, crenation, agglutination, recovery, and cyst formation) during exposure to normal and immune rabbit serum, provided a definitive assay for the response of rabbits to injected amoebae. A large variation in the immobilization titers among three rabbits during a period of 90 days after injection with A. castellanii (Neff strain) was observed.
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