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Abstract

 The recombinant antibodies AC650, AC653, AC656 and AD460 detect the human CD8α protein by flow cytometry.
Highlights
CD8 protein, composed of two subunitsa( and b), is a transmembrane glycoprotein complex expressed primarily in cytotoxic T lymphocytes (Parnes, 1989)
We describe the ability of four recombinant antibodies (AC650, AC653, AC656 and AD460) to successfully detect the CD8a protein (Uniprot P01732) in CD8atransfected HEK293 cells
Medecine/antibodies/) as mini-antibodies with the antigenbinding portion fused to a rabbit IgG Fc
Summary
CD8 protein, composed of two subunitsa( and b), is a transmembrane glycoprotein complex expressed primarily in cytotoxic T lymphocytes (Parnes, 1989). 1 Bachelor in Biomedical Sciences, Faculty of Medicine, University of Geneva, 1 rue Michel Servet, CH-1211, Geneva, Switzerland 2 Geneva Antibody Facility, Faculty of Medicine, University of Geneva, 1 rue Michel Servet, CH-1211, Geneva, Switzerland 3 University Clinics of Dental Medicine, University of Geneva, 1 rue Michel Servet, CH-1211, Geneva, Switzerland 4 Flow Cytometry Core Facility, University of Geneva, 1 rue Michel Servet, CH-1211 Geneva, Switzerland The recombinant antibodies AC650, AC653, AC656 and AD460 detect the human CD8a protein by flow cytometry.
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