Abstract
This research was conducting to quantify the berberine concentration. Rapid method using 1H-NMR was used to determine berberine quantitative and qualitatively from the crude extract. Tali kuning was collected from Manokwari Papua Barat and powdered with hammer mill. Sonication was employed to extract using methanol at room temperature. 500 µg authentic berberine chloride were dissolved in 5.4 mL methanol-d4 (containing 84.4 µg anthracene). 1H-NMR spectra were recorded in methanol-d4 (99,9%) using JEOL JNM-ECX 500. Each sample was scanned for 100 using the following parameter 0.18 Hz/point, spectral width 14400 Hz, pulse with 4.0 US, relaxation delay 2 sc. Peak areas were used for qualitative analysis and integration of each peach were employed for quantitative analysis. The results demonstrated that 1H-NMR signal pattern of H-13 and H-8 recorded from Tali Kuning, and Amur corktree were well recorded, and in accordance to the berberine chloride standard. Using peak integration of H-13 and H-8, the berberine quantity in Tali kuning is 18.06 mg/g of dried powder, and 22.78 mg//gr for Amur corktree. Berberine percentage based on the weight of oven-dried-extracts was 8.34% (MC 7.54%) and 12.04% (11,54%) for Amur corktree and Tali kuning.
Highlights
Several methods for quantitative and qualitative analysis of bioactive compounds in medicinal plants have been reported by several researchers, ranging from conventional methods, such as column chromatography (CC), high performance liquid chromatography (HPLC), high performance displacement liquid chromatography (HPDC) to advanced methods, like 1H-NMR, and liquid chromatography (LC) with electron impact or electrospray ionization mass spectrometry (EI/ESI-MS)
The results of rapid determination of berberine in two medicinal plants using 1H-NMR are shown in Fig. 1(A, B, and C)
Whereas the 1H-NMR spectra of berberine recorded for the crude extracts
Summary
Several methods for quantitative and qualitative analysis of bioactive compounds in medicinal plants have been reported by several researchers, ranging from conventional methods, such as column chromatography (CC), high performance liquid chromatography (HPLC), high performance displacement liquid chromatography (HPDC) to advanced methods, like 1H-NMR, and liquid chromatography (LC) with electron impact or electrospray ionization mass spectrometry (EI/ESI-MS).Advanced analytical instruments, such as 1H-NMR and EI/ESI-MS etc,to determine quantities and qualities of QPAs in medicinal plants directly with extracts have been reported and employed by several researchers. Li et al (2006; 2009) reported that rapid and simple determinations of protoberberines in the two most famous Chinese medicinal herbals, Cortex phellodendri (Huangbai) and Rhizomacoptidis (Hunglian), using 1H-NMR were successfully achieved.The 1H-NMR spectra of berberine, mainly proton signals of H-13 and H-8 at ring C appeared as singlet on the specific spectrum regions (non crowded region) at 8.69 – 9.79 ppm, where is no interference from other signals occurred (Li et al, 2006; 2009). Persentase kandungan berberin didasarkan pada berat ekstrak kering oven adalah 8,34% (kadar air 7,54%) dan 12,04% (11,54%) untuk Amur corktree and Tali kuning. This figure illustrates that proton signals of H-13 of berberine and palmatine appear at 8.69 and 8.79 ppm, respectively, where both resonateat non crowded-region.
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