Abundance of T Stem Cell Memory in F-CAR-T Cells CausingYounger Phenotypes

Abstract

This study will introduce a novel anti-CD19 CAR-T technology – FasT CAR-T (F-CAR-T)- shortens production time,increases production quality, and produces healthier T cells with a younger phenotype. This study also investigates theeffect of the abundance of T stem cell memory in F-CAR-T cells’ correlation with younger phenotypes, leading to betterpersistence in T cells by manipulating the number of Car T cells injected into Raji xenografts. Using mouse cell lines,RAJI xenograft models, and F-CAR-T cells, this study will measure cell proliferation of Tscm, CD45RO-, CD62L+,and PD1/Lag3/Tim3 by flow cytometry and count Car expressing cells using SCFV antibodies. The experiment resultswill show if F-CAR T cells have significantly greater expansion and T stem cell memory than C-CAR-T cells. TheC-CAR-T cells with less exhaustion, indicating the effect of T stem cell memory distinguishing F-CAR-T therapy fromconventional CAR-T therapy. The result of this study will provide critical information for the future clinical trial ofF-CAR-T cell therapy. This work can inspire to utilize the T stem cell memory, which exhibits great antitumor activity,to explore the mechanism of modulation of CAR-T cell metabolism and T cell differentiation.