Abundance and diversity of the culturable nectar mycobiome in Rhododendron catawbiense varies with elevation

Abstract

Abstract The nectar microbiome can influence pollinator choice and plant fitness. Previous research has shown that changes in environmental conditions at large spatial scales can influence nectar microbiome composition. However, little is known about how changes in climate with increasing elevation affect nectar microbiome abundance and composition. Here we describe the culturable nectar mycobiome of Rhododendron catawbiense (Ericaceae) by quantifying colony abundance, identity, and richness of fungal genera. We further evaluate how the culturable nectar mycobiome (CNMB) abundance, diversity, and composition (i.e., the fungal species within the nectar microbiome) varies at two different elevations. Nectar samples were collected from R. catawbiense individuals at a high and low elevation and were cultured on yeast agar with 0.01% chloramphenicol media. Fungal colonies were categorized morphologically, quantified, and then identified using DNA barcoding. In total, 2,822 fungal colonies were recorded belonging to six genera across both elevations. Elevation did not influence CNMB diversity (Simpson’s diversity index) or genera richness per flower, however only three genera were found at the high elevation while six were found at the low elevation. Elevation had a significant effect on colony abundance with a 95% increase in the number of colonies in nectar samples at low compared to the high elevation. Variation in abundance and overall genera composition of fungal colonies across elevations may have the potential to affect nectar quantity and quality and ultimately pollination success. This study adds to our understanding of the drivers of CNMB composition across spatial scales its potential implications for plant-pollinator interactions.