ABSTRACTS OF POSTER PRESENTATIONS

Kazuya Kudoh ,Yoji Yamazaki ,Zbigniew Darżynkiewicz ,Kouichi Satou ,Masaaki Tachibana ,Sanshiro Okamoto ,Shigeya Kyouda ,Hasegawa Etsuko ,Kentaro Fujii ,Eigo Sato ,Masakazu Akahori ,Ai Kazami ,Junichi Kaganoi ,Hiroshi Mizuhara ,Hisashi Hashimoto ,Hiroyuki Takahashi ,Takuya Iida ,Kazutoshi Shibuya ,Masayuki Soma ,Akira Harashima ,Ciko Ishiwata ,Go Watanabe ,Kenji Sakoda ,Katsuhiko Yanaga ,Yutaka Tagawa ,Soh Sato ,Takehiko Yoshimoto ,Yoichi Negishi ,Hiroyuki Sasaki ,Hiroshi Itoh ,Kazushige Kiguchi ,Hajime Ishikawa ,Eizo Kimura ,Makoto Ohori ,Yoshifumi Mori ,Makoto Yasuda ,Yoshihisa Yano ,Hans Drexler ,Koichi Sasaki ,Motoyoshi Yamamoto ,Yuri Akishima ,Tsuyoshi Fukushima ,Hiroyuki Tanaka ,Koji Hosaka ,Makoto Sonobe ,Kobayashi Yohichi ,Watanabe Mari ,Yuichi Izumi ,Junko Sunaga ,Kohei Nakata ,Kazushige Kiguci ,Naoki Okamoto ,Ayumi Okochi ,Ohhara Tazuru ,Satoru Motoyama ,Masayuki Imamura ,Dorota Halicka ,Junko Hirata ,K. Okayasu ,Takeshi Kawamura ,Teiichirou Aoyagi ,Yusuke Okuda ,Satoshi Ohi ,Tadashi Hatano ,Piotr Smolewski ,Masashi Kimura ,Yoji Sato ,Yoshiro Sato ,Nobuhiro Deguchi ,Ryoko Tomimai ,Yoshio Ohno ,Ohkuma Yoshiki ,Hirokazu Kashiwagi ,H. Kubo ,Chieko Ishiwata ,Isamu Ishiwata ,Masato Kondo ,Mamoru Kobayashi ,Keiko Enomoto ,Kazunori Namiki ,Haruo Hashimoto ,Shinji Hirotsune ,Yoshihiro Nakagami ,Koki Nagaike ,Kumiko Tsuboi ,Kazuki Ueda ,Shiro Nagatani ,Masamichi Ita ,Hideyuki Motohashi ,Yoshiharu Akasaka ,Hiroaki Kataoka ,Yoshihiro Kikuchi ,Tadao Tanaka ,Toshihide Nishimura ,Yuichi Ishida ,Seigo Ohi ,Shinzo Kitahara ,Hitoshi Kubo ,Kazuyo Kohama ,Tatsuo Gondo ,Hiroshi Ishikawa ,Hiroshi Nakaya ,Mayumi Ishikawa ,Noriyuki Yoshida ,Shöichiro Tsukita ,Ginko Osawa ,Toshiaki Tachibana ,Masako Shimojo ,Bunpei Ishizuka ,Isao Tabei ,Shoji Saito ,Mayuko Matumoto ,Noboru Sakamoto ,Yuko Tokieda ,Masako Iino ,Akihiko Misawa ,M. Yamamoto ,Kazuhito Matsushita ,Haruhiro Kondo ,Gen Yoshino ,Masashi Takano ,Tomoharu Tamagawa ,Toru Kita ,Hiroyuki Kato ,Ryusuke Ito ,Yasushi Iida ,Yosuke Kanishi ,Kahei Sato ,Kazuhiro Kimura ,Masaru Okabe ,Shigeki Niimi ,Hisahiro Kamoi ,Takashi Yokose ,Kunihiko Yoshioka ,Hajime Ueshiba ,Yoshinobu Matsuo ,Kyosuke Yamada ,Munehisa Ueno ,Hashimoto Hisashi ,Megumi Iguchi ,Kunzo Orita ,Hiroo Sekiya ,Kazumi Kamoi ,Tomoyuki Okumura ,Sonshin Takao ,Shuichiro Uchiyama ,Atsushi Okamura ,S Koga ,Nagazumi Suzuki ,Sato Soujiro ,T. Ohkubo ,Yutaka Shimada ,Tomoka Wachi

doi:10.1111/j.1749-0774.2004.tb00078.x

Abstract

s of poster presentations 44 P010 Antifungal agents induces alteration in the matrix composition of candida glabrata biofilms Rodrigues, Celia (Minho University, Departement of Biological Engineering Department, Braga ); Fonseca, Elza (Minho University, Departement of Biological Engineering Department, Braga , AUT); Goncalves, Bruna (Minho University, Departement of Biological Engineering Department, Braga ); Bogas, Diana (Minho University, Departement of Biological Engineering Department, Braga ); Silva, Sonia (Minho University, Departement of Biological Engineering Department, Braga ); Azeredo, Joana (Minho University, Departement of Biological Engineering Department, Braga ); Henriques, Mariana (Minho University, Departement of Biological Engineering Department, Braga ) Candida glabrata has emerged as the second most prevalent pathogen, after Candida albicans, in mucosal and invasive fungal infection. Its ability to form biofilms has been considered one of the most important virulence factors, since they present a high resistance to antifungal agents used in fungal infections treatment. Moreover, there is a lack of information about the physiological response of C. glabrata biofilms to antifungal agents. Thus, the aim of this study was to evaluate the effect of different antifungal agents on C. glabrata biofilm composition and the influence in related resistance genes expression. For that C. glabrata biofilms were formed in the presence of fluconazole (Flu), voriconazole (Vrz) and amphotericin B (AmB). Biofilm matrix composition was evaluated in terms of polysaccharides, proteins and ergosterol and ERG genes expression was also assessed. As expected C. glabrata biofilms are more resistant to Flu, Vrz and AmB than planktonic cells. . Although in a strain dependent manner, polysaccharides were increased in the presence of the antifungals, in opposition to proteins, which decreased in the presence of AmB and Vrz. Due to the interaction of these agents with ergosterol, even in different ways, we evaluated, for the first time, the presence of this compound in the extracelluar matrix. It was noticed that ergosterol was, in fact, present in all the matrices and in general it increased with the presence of the drugs. Therefore, there is an obvious answer of biofilm cells to the stress induced by the different agents, that caused and alteration of matrix composition. In order to determine if the increased concentration of ergosterol in the biofilm matrix was caused by an up-regulation of proteins responsible for ergosterol synthesis, ERG expression was evaluated.. Although ERG gene expression was very strain dependent is was possible to verify that some genes, as ERG11 and ERG6, were upregulated in biofilm cells. Interestingly one strain was unable to express ERG genes when grown in the presence of Vrz. It was then possible to conclude that biofilm cells upon exposure to antifungal agents overexpress ERG genes, which seems to contribute to an increase in ergosterol concentration in the biofilm matrix.

Highlights

The fungal disease of great medical importance due to mortality and morbidity it causes, cryptococcosis, has a strong association between the incidence and the presence of HIV, lymphoproliferative disorders or immunosuppressive therapies
Four Aspergillus species namely, A. flavus, A. parasiticus, A. niger and A. terreus were plated on Rose Bengal Agar media and morphologically identified
To determine the natural seed infection by Aspergillus spp. and other fungi, undamaged pods from the middle two rows were carefully handshelled and 100-seed of each plot were surface sterilized by soaking in 5% aqueous solution of sodium hypochlorite (NaOCl) for 3 minutes and immediately rinsed with sterile distilled water, and plated on Czapek-Dox agar medium and incubated at room temperature

Summary

Introduction

The fungal disease of great medical importance due to mortality and morbidity it causes, cryptococcosis, has a strong association between the incidence and the presence of HIV, lymphoproliferative disorders or immunosuppressive therapies. Invasive fungal diseases (IFDs) are an important cause of morbidity and mortality in patients undergoing allogeneic stem cell transplantation (SCT). The aim of this study is to investigate the performance of Aspergillus-LFD as a point-of-care test for the diagnosis of invasive fungal infections (IFI). Invasive fungal infections (IFI) are important causes of morbidity/mortality in immunocompromised patients with hematologic malignancies. Incidence rates of invasive fungal infections (IFI) of >10% and case fatality rates in the order of 50% justify systemic antifungal prophylaxis (SAP) in children and adolescents with acute myeloid leukemia (AML) and recurrent acute leukemia (RAL). Algorithms for neutropenic fever or signs and symptoms of infection include monitoring with blood cultures, high resolution computed tomography (HR-CT), and, in patients with lung infiltrates, serial serum galactomannan, invasive diagnostics and preemptive therapy with change in class. There were no significant changes in immunological and radiological outcomes

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