Abstract
Abstract The injection of 20 µg of a highly purified preparation of human C′2 mixed with complete Freund's adjuvant into each popliteal lymph node of a rabbit, followed a month later by intramuscular injection of 40 µg of C′2 mixed with adjuvant, elicited a highly potent antiserum to C′2. The antibody inhibited the hemolytic activity of C′2, both in free solution and in cell-bound form, and produced a single precipitin line with whole human serum and with purified C′2 by Ouchterlony test. The two lines gave a reaction of identity. On immunoelectrophoresis, using agar as a supporting medium, purified C′2, which on Pevikon electrophoresis is a β globulin, was found to have an electrophoretic mobility almost equal to that of albumin. However, in the presence of purified C′4, and likewise in whole serum, its mobility was reduced to that of a β globulin, presumably due to C′2 to C′4 interaction.
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