Abstract
Abstract Sheep red cells can be of high or low potassium concentration, which is under genetic control. It was proposed that the difference in potassium concentration depends on the level of membrane Na-K dependent ATPase (Tosteson, D. C., Fed. Proc., 22: 19, 1963). Experiments were performed to ascertain whether high and low potassium sheep red cells could be employed as a model to study the participation in immune hemolysis of cell, or cell membrane factors. High and low potassium red cells were treated with constant amounts of rabbit antibody and guinea pig complement for 1 hr at 37°. When the concentration of these reagents was adjusted to obtain 45.0% (±7.0) lysis of low potassium erythrocytes, only 20.7% (±3.6) lysis was obtained with high potassium erythrocytes. By contrast, osmotic fragility was similar in both types of cells. Several methods, including immune adherence, showed that the high and low potassium erythrocytes reacted similarly with antibody and with the complement components C′1, C′2, C′3, and C′4.
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