ABSTRACTS: 7 Study of uterine spiral arteries during implantation window in women with normal fertility or implantation failure

Abstract

Problem: Vascular remodelling and angiogenesis are essential for effective human implantation. Clinical tests measuring plasmatic angiogenic factors tend to predict complications of impaired implantation; however, the role of these factors in the endometrium at the time of implantation is not established and has been investigated.Material and Methods: On patients with normal fertility or implantation failures, we performed pelvic 3‐D ultrasound and endometrial biopsy during mid‐luteal phase. Endometrial volume, resistance of uterine arteries and sub endometrial blood flow were quantified by ultrasound. For each biopsy, one sample was used for histological dating, CD56+ count, morphological study of vessels and immunohistochemistry (VEGF, VEGF‐R1, VEGF‐R2). Another sample underwent RNA extraction and RT‐PCR (angiogenic factors).Results: We noticed that our data (morphology, immunohistochemistry, profile of pro‐angiogenic expression) tend to be different between our two groups.Conclusion: These results may help establishing new criteria to define optimum uterine receptivity.