Abstract
Introduction: Stem cell therapy is one of the most promising strategies for treating intracerebral hemorrhage (ICH). Human mesenchymal stem cell (MSC) is a frequently used cell source, however, it is not perfect. The main weakness of MSCs is that they gradually lose the stemness during in vitro expansion (usually fewer than P10 in subculture), which limits their availability in clinical application. We developed a method to isolate MSCs from human term placenta in a serum-free selective culture condition and named these cells as placenta choriodecidual membrane-derived MSCs (pcMSCs). According to our in vitro data, pcMSCs expressed standard MSC cellular markers more than P20 during in vitro expansion. Objective: To evaluate whether pcMSC improves neurological deficits in a rat model of ICH and study the underlying mechanisms. Methods: The ICH rat model was induced by striatal injection of collagenase type VII. One day after ICH induction, pcMSCs were directly injected into the hematoma site. Behavior tests such as rotarod test were repeatedly performed for four weeks. [18F]FEPPA positron emission tomography (PET) and magnetic resonance imaging (MRI) were applied to reveal neuroinflammation and brain atrophy, respectively. Results: The rats in situ injected with pcMSCs showed better performance in the rotarod test which assesses the motor coordination and balance (see Figure). Such neurological recoveries occurred four weeks after treatment. MRI data showed reduced brain atrophy in pcMSC-treated rats at the end of study, while preliminary data of PET suggested smaller signals within one week. Conclusion: Human pcMSCs improved the neurological deficits in ICH rats. The underlying mechanisms may be the reduced neuronal loss (indicated by reduced brain atrophy) associated with the regulated neuroinflammation (indicated by smaller signals). Currently, the therapeutic effects of pcMSCs to ICH rat model are intensively studied.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
