Abstract TP240: The Change Of Motor Functions After Post-stroke In 5xfad

Abstract

Background and Rationale: Recent Alzheimer’s disease (AD) studies with 5XFAD, a mouse model of Alzheimer’s disease, were mainly focused on cognitive functions. Here, to investigate the change of motor functions after post-stroke in the AD mouse model, we generated the 5XFAD mice and examined their long-term behavior changes. Methods: To determine the role of 5XFAD after post-stroke, we generated the 5XFAD mice, induced the Ischemia/Reperfusion (30-minute middle cerebral artery occlusion (MCAO)/14 days reperfusion after 30-minute MCAO induction) injury model in mice, and tested the several behavior effects of 5XFAD (2 months vs. 6 months). Relative cerebral blood flow was measured by Laser Doppler. We daily investigated neurological deficit score, wire-hanging test with both time latency and grip score, and cylinder test for 14 days. Results: We found that CBF changes and brain shrinkages at 14 days after post-stroke were not changed in 5XFAD mice compared to the littermate control. The daily locomotor changes of neurological deficit score for 14 days were not significantly worsened compared to the littermate control. The Cylinder test shows that locomotor abnormalities were not changed in 5XFAD mice. The Wire-hanging test shows that locomotor abnormalities both latency and grip scores were worsened in 5XFAD mice compared to littermate control. Conclusion: These findings indicate that the long-term abnormalities of locomotor in 5XFAD mice may be an important therapeutic target for post-stroke in AD.