Abstract TP108: 2,2 Dipyridyl, An Iron Chelator, Does Not Reduce Intracerebral Iron Toxicity Or Improve Outcome After Intracerebral Hemorrhagic Stroke In Rats

Abstract

Background — After an intracerebral hemorrhage (ICH) iron is released from degrading erythrocytes over days, which causes secondary damage by increasing free radicals. Many studies show that iron chelators, such as deferoxamine, lessen injury, but not all studies support this. Hypothesis — The ferrous iron chelator, 2,2 Dipyridyl (DP), will decrease injury after ICH or intraparenchymal FeCl 2 infusion in rats. Experiment 1— Rats were given a collagenase-induced striatal ICH. In experiments 1 and 3, we tested whether behavior was improved (e.g., walking) from administering DP (25mg/kg/day, 12 hours after surgery for 3 days). They were euthanized after 7 days to determine non-heme iron levels in the brain. Experiment 2— Rats were injected with DP (20mg/kg) 6 hours after collagenase infusion and every 24 hours till euthanasia at 3 days for measuring edema. Experiment 3— After injecting FeCl 2 in the striatum rats were given DP (25mg/kg every 12 hr starting 2 hours prior to surgery for 3 days). The volume of tissue loss and Fluoro-jade staining (degenerating neurons) was measured. Results — DP did not improve behavioral or histological outcome or reduce edema in either the collagenase ICH or FeCl 2 model. DP also did not affect parenchymal non-heme iron level. Conclusion — Our data suggests that DP on its own is not an effective strategy for ICH.