Abstract PS14-28: Core−shell structured gold nanorods@iron-based metal-organic framework for magnetic resonance imaging guided photothermal therapy in triple-negative breast cancer

Abstract

Abstract Background/Objective: As the most aggressive subtype of breast cancer, triple-negative breast cancer (TNBC) with high mortality lacks effective treatment options and results in poor prognosis. Photothermal therapy (PTT) has emerged as a potential anticancer strategy. Until now, the construction of multifunctional photothermal agents is vital importance for effective PTT generally. In this work, the novel core-shell gold nanorods (GNs)@iron-based metal-organic framework (Fe-MOF) was firstly successfully fabricated for magnetic resonance imaging (MRI) guided PTT in TNBC. The inner GNs have excellent photothermal properties and the outer Fe-MOF shell endow the nanocomposite with superior T2 MRI property.Methods: The GNs @ Fe-MOF was firstly prepared by a layer-by-layer technique. Prepared nanocomposite was characterized by measuring measuring measuring transmission electron microscope (TEM), UV-Vis-NIR absorption spectra, powder X-radiffraction (XRD) patterns. MR imaging of nanocomposite was assessed in vitro and in vivo. The biocompatibility and photothermal cytotoxicity of the nanocomposite was assessed by a CCK-8 assay. The photothermal effect of nanocomposite in vivo was evaluated by monitoring tumor growth. The tumor tissues were analyzed by Hema-toxylin and eosin (H&E) to further analyze photothermal effect. The toxicity of the materials in major organs was also investigated by H&E.Result: The core-shell GNs @ Fe-MOF was successfully prepared and displayed excellent dispersion. The nanocomposite possessed good biocompatibility and effectively killed tumor cells under laser irradiation. Meanwhile, the nanocomposite showed clear signal contrast in T2-weighted images in vitro and in vivo. Moreover, the nanocomposite significantly inhibited tumor growth under laser irradiation in vivo. H&E showed that the nanocomposite caused serious damage to tumor tissue under laser irradiation.Conclusion: The current work developed a novel multifunctional core-shell GNs @ Fe-MOF with good biocompatibility for MRI guided PTT in TNBC.Keywords : Magnetic resonance imaging, Photothermal therapy,gold nanorods@iron-based metal-organic Framework Citation Format: Jingchao He, Haijun Zhang. Core−shell structured gold nanorods@iron-based metal-organic framework for magnetic resonance imaging guided photothermal therapy in triple-negative breast cancer [abstract]. In: Proceedings of the 2020 San Antonio Breast Cancer Virtual Symposium; 2020 Dec 8-11; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2021;81(4 Suppl):Abstract nr PS14-28.