Abstract PR456

Abstract

Background & Objectives: Genetic material has been shown to be a sensitive target of numerous harmful agents including anesthetics which may entail various adverse health effects in patients as well as in operating room personnel. Knowledge about their genotoxic effects can give valuable support to anaesthesia care providers to make better treatment performance and improve patient safety. The present study was aimed to assess and compare the genotoxicity of three intravenous agents (propofol, thiopental and ketamine) that were used for induction during balanced anaesthesia technique with inhalational anaesthetic isoflurane in patients undergoing elective surgery. Materials & Methods: A total of 18 adult patients, aged 18-50 years, underwent elective abdominal procedures lasted about 120 minutes were selected for this study. They were randomly divided into 3 groups (n=6) receiving propofol, thiopental and ketamine induced balanced anaesthesia respectively. In all groups anaesthesia was maintained with isoflurane inhalation. Intravenous samples of blood were collected before induction of anaesthesia, and immediately 10, 60, 120 minutes, 6 and 12 hours after induction of anaesthesia. Lymphocytes were separated and comet assay was performed in which lymphocytes suspension on agarosed slides was lysed in salts and detergents containing lysing solution, exposed to alkaline buffer solution for DNA unwinding and then following electrophoresis at 24 volts and 300 mA, was stained with ethidium bromide. Lymphocytes were analyzed under fluorescent microscope to evaluate genotoxicity. Results: 50 cells per sample per patient were evaluated, categorized as undamaged to highly damaged (class0-class3) cells and damage to DNA was measured as damage index. In all study groups, damage index start increasing after induction until the end of surgery and maximum damage was seen after 120 minutes of anaesthesia induction as 37.17±4.99, 63.50±4.85 and 66.20±7.56 for propofol, thiopental and ketamine groups respectively. However this damage started to decrease after 6 hours. Conclusion: By comparing the three IV induction agents, it was concluded that propofol balanced anaesthesia with isoflurane showed the least or no gonotoxicity. Disclosure of Interest: None declared