Abstract PR096

Abstract

Background & Objectives: Sepsis is a leading cause of mortality and critical illness[1]. ALI/ARDS remain common complications of sepsis, which is intimately connected with the structure and function of mitochondrion[2]. Hydrogen gas (H2) exerts significant role in the treatment of sepsis[3]. Objective to evaluate the effects of H2 on mitochondrial function and morphology in sepsis-induced acute lung injury mice Materials & Methods: 104 male ICR mice, weighing 20-25g and aged 6 weeks, were randomly divided into 4 groups (n=26 each): sham operation group (Sham group), Sham+H2 group, sepsis group and Sepsis+H2 group, Sepsis was produced by cecal ligation and puncture (CLP). The mice in the Sham+H2 and Sepsis+H2 groups inhaled 2% H2 for 1 h starting from 1h and 6 h after Sham and CLP operation. The survival rates of animals in all groups were measured. At 24h after operation, the arterial blood gas analysis was conducted, the pulmonary specimens were obtained to determine the pathological changes of lungs were scored. Electronmicroscope was used to observe ultrastructural changes. Lung mitochondrial respiration control rate was test by clark eletron. Mitochondrial membrane potential was determined using JC-1 fluorescent probe. ATP synthesis capacity was determined using a bioluminescence technique. Results: H2 treatment increased the 7d survival rate of severe CLP mice from 0% to 60% (vs. Sepsis group, P<0. 05). Compared with Sham group, the pathological scores were elevated, PaO2/FiO2 was decreased in group Sepsis (P<0.05). Compared with Sepsis group, the pathological scores was decreased, PaO2/FiO2 were elevated in group Sepsis+H2(P<0.05).Electrorunieroseope showed the morphology of the lung mitochondria in group Sepsis significantly changed(such as swelling, disordered arrangement, crest fracture and so on). Compared with Sham group, lung mitochondria of RCR, MMP, ATP expression were decreased in group Sepsis (P<0.05). Compared with Sepsis group, RCR, MMP, ATP expression were increased in group Sepsis+H2 (P<0.05). Conclusion: Hydrogen reduces acute lung injury in septic mice via moderating mitochondrial dysfunction.