Abstract PR010: Multiplex imaging reveals features of organotropism in pancreatic ductal adenocarcinoma

Abstract

Abstract PDAC patients have a dismal prognosis with an 11% 5-year survival rate, driven in part by the fact that 90% of patients are diagnosed with metastatic disease. In a cohort of 300 PDAC patients, we observed that patients who had lung-only metastasis had longer overall survival than the majority of patients with metastasis to the liver. We used gene expression data to develop a signature based on gene expression in primary tumors of liver versus lung organotropism, which we called pORG. Our pORG signature predicts long term survival in PDAC patients and reveals unique features of the primary tumor, including increased interferon and immune activity in lung tropic tumors and increased proliferation and replication stress in liver-tropic tumors. To validate our findings, we generated cyclic immunofluorescence multiplex imaging data for a subset of 34 patients. Single cell image analysis revealed B and T cell infiltration and spatial clustering in low pORG/ lung tropic tumors, and markers of replication stress in high pORG patients. Our work suggests unique targetable tumor and microenvironment features linked to organotropism in PDAC. Citation Format: Jennifer Eng, Jason Link, Carl Pelz, Rosalie Sears. Multiplex imaging reveals features of organotropism in pancreatic ductal adenocarcinoma [abstract]. In: Proceedings of the AACR Special Conference: Cancer Metastasis; 2022 Nov 14-17; Portland, OR. Philadelphia (PA): AACR; Cancer Res 2022;83(2 Suppl_2):Abstract nr PR010.