Abstract
Abstract Cancer stem cells (CSCs) are suggested to be responsible for drug resistance and aggressive phenotypes of tumors. Mechanisms that regulating CSCs are still under investigation. Our lab has established a novel method to produce CSCs from induced pluripotent stem cells (iPSCs) under cancer microenvironment using conditioned medium (CM) from cancer cell lines. By treatment iPSCs with CM, cells gain CSC characteristics. Here, we analyzed transcriptome from CSCs, which converted from iPSCs using CM from pancreatic ductal adenocarcinoma cells, and differentially expressed genes were identified. Pathway enrichment was analyzed by the Kyoto Encyclopedia of Genes and Genomes (KEGG). Comparing iPSCs with CSCs converted from iPSCS showed elevated expression of genes related to ERBB2, ERBB3 and PI3K, while KEGG pathways revealed enrich of pathways known to be involved in cancer including ERBB2/ERBB3 signal pathway by conversion of iPSCs to CSCs. Inhibition of ERBB2 by lapatinib induced cell proliferation arrest and losing of tumorgenicity of converted cells. This study shows a potential involvement of ERBB2/ERBB3 pathway in CSCs generation and could lead to potentially new options for cancer treatment and prevention. Citation Format: Ghmkin Hassan, Masaharu Seno. The conversion of induced pluripotent stem cells into cancer stem cells under pancreatic cancer microenvironment is inhibiting by lapatinib [abstract]. In: Proceedings of the AACR Virtual Special Conference on Pancreatic Cancer; 2020 Sep 29-30. Philadelphia (PA): AACR; Cancer Res 2020;80(22 Suppl):Abstract nr PO-037.
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