Abstract P6-05-05: The expression of lysyl oxidase and fibrotic focus is related to inflammation in breast cancer

Abstract

Abstract Background: Lysyl oxidase (LOX) is an extracellular matrix enzyme that catalyzes the cross-linking of collagens or elastin. Our hypothesis is that LOX contributes to the formation of a fibrotic focus (FF), which is related to inflammation in breast carcinogenesis. In this study, we analyzed the association between the expression LOXs and FF, and investigated prognostic significance in breast cancer. Methods: Tissue microarrarys were constructed from the specimens of 444 patients with primary invasive breast cancer. Immunohistochemical staining for LOX, LOXlike (LOXL)-, LOXL-2 and LOXL-3 was performed. The status of FF within the tumor was assessed. The number of CD4+ T cells, CD8+ T cells, CD68+ macrophages was counted, and intratumoral and peritumoral lymphocyte infiltration were evaluated. The clinicopathologic characteristics of the patients were analyzed. Result: The percentage of positive FF was 39.2% and positive rate of LOX expression was 50% in primary breast cancer tissues. FF was found to be significantly associated with intratumoral and peritumoral inflammation, lymph node metastasis, high histologic grade, larger tumor size. LOX was associated with intratumoral and peritumoral inflammation, CD8+ T cells and menopausal status. LOXL-3 was significantly associated with positive expression of ER and PR, and molecular subtype. Conclusions: FF and the expression of LOX were associated with inflammation in breast cancer in this study. Our results suggest that LOXs may contribute to the formation of a FF indirectly in relation with inflammation in breast cancer. Further studies are needed to clarify the role of LOXs, FF and inflammation in tumorigenesis and prognostic value of them in breast cancer. Citation Format: Park SH, Jeong YJ, Bong JG, Oh HK. The expression of lysyl oxidase and fibrotic focus is related to inflammation in breast cancer [abstract]. In: Proceedings of the 2016 San Antonio Breast Cancer Symposium; 2016 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2017;77(4 Suppl):Abstract nr P6-05-05.