Abstract P426: Quercetin Intake Differentially Affects the Plasma Lipid Levels and Lipoproteins Associated Gene Expression in Normal C57bl Female Mice

Abstract

Background: Quercetin is a member of the bioflavonoids family known to have antiatherogenic, antiinflammatory, and antihypertensive properties; and reported to have cardiovascular protective role. Quercetin through its antioxidant action may protect against oxidative stress induced by free radicals consequently decreasing the potential for modification of low density lipoprotein cholesterol (LDL); however it is not clear if the intake of quercetin during exercise may have beneficial effect on plasma lipids. Goals and hypothesis: We hypothesized that the intake of quercetin with exercise will affect plasma lipids. The goal of this study is to investigate the beneficial effect of Quercetin on normal female mice. Study Design and Methods: We investigated the possible beneficial effects of the combined exercise and quercetin on plasma lipids in C57BL6 normal female mice fed normal mouse chow. Mice (N=29) were divided into four groups. These groups are as follows: Control mice, left untreated; control quercetin group, orally supplied with 100 μg/day of quercetin without exercising; exercise group without quercetin, and exercise group with quercetin supplements. The exercise groups were run on a treadmill for 30 minutes, 15m/m/ 5 days/week for 30 days. At the end of the month of the treatment, mice were sacrificed liver gene expressions for genes associated with lipoprotein metabolism were analyzed, and plasma lipids levels were analyzed. Results and Discussion: Our data interestingly showed that Quercetin differentially influences the levels of lipoprotein, oxidative stress and inflammatory markers gene expression during exercise in normal female mice; however plasma lipids, specifically TG plasma levels have elevated among mice consuming quercetin alone or with exercise significantly (P<0.5). Conclusion: This study have demonstrated that Quercetin intake with exercise may differentially affects the plasma lipid levels and lipoproteins associated gene expression in normal C57BL female mice.