Abstract P4-01-18: The relationship between components of tumour inflammatory cell infiltration and hematogenous metastasis in patients with primary breast cancer

Abstract

Abstract Background: Tumors are infiltrated by inflammatory cells (e.g. macrophages, B and T lymphocytes), which may promote tumor growth and invasion. However, the clinical correlation between hematogenous metastasis and relative amount of inflammatory cells is still unknown. Methods: The prevalence of circulating tumor cells (CTC) in peripheral blood of 133 breast cancer patients was detected by a multimarker comprised of cytokeratin 19 (CK19), small breast epithelial mucin (SBEM) and human mammaglobin (hMAM) real time quantitative RT-PCR (QPCR) platform. Meanwhile, CD68+macrophages, CD20+B lymphocytes and CD3+T lymphocytes at the peritumor and intratumor were also counted by immunohistochemical examination, in corresponding primary invasive breast carcinomas. The relationship between CTC in peripheral blood and inflammatory cell infiltration was analyzed. Results: The positive rate of CTC was 39.8% (53 out of 133). The prevalence of CTC in peripheral blood was only observed in invasive breast carcinomas. There were more inflammatory cells at the peritumor than the tumor center (P < 0.001).In addition, higher total numbers of infiltrating both CD68+macrophages and CD3+T lymphocytes were associated with significantly the prevalence of CTC in peripheral blood (P < 0.05). Conclusion: Our data support a hypothesis that CD68+macrophage and CD3+T lymphocyte infiltrating in the breast cancer microenvironment may promote tumor cells disseminating through circulation system. Citation Format: Naping Wu, Jue Wang, Tiansong Xia, Wenbin Zhou, Zhao Liu, Yi Zhao, Xiaoan Liu, Xiaoming Zha, Shui Wang. The relationship between components of tumour inflammatory cell infiltration and hematogenous metastasis in patients with primary breast cancer [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P4-01-18.