Abstract P2-07-08: Interaction of mesenchymal stem cell with breast cancer lineage cells and evaluation of their biological behavior

Abstract

Abstract Breast carcinoma is a highly prevalent and incident disease. The interaction between tumor stroma and malignant epithelial cells has been reported as a mechanism of resistance to cytotoxic treatment. Mesenchymal cells originating from the tumor stroma create a favorable microenvironment for cancer stem cells (CTTs) maintenance. CTTs are able to repopulate the host with tumor cells of the same origin on distant sites. It is postulated that this interaction modulates the ability of tumor to invade and disseminate. The aim of this study is to analyze the association MCF-7 cell line with human mesenchymal stem cells (MSC), and evaluate the biological behavior and phenotypic changes. MSC cells derived from Wharton's jelly were co-cultured with MCF-7. Cell migration assay: MSC cells were seeded into 12 well plates and co-cultured with MCF-7 cells in the proportions 1% MSC + 99% MCF-7, 10% MSC + 90% MCF-7, 30% MSC + 70% MCF-7 and 100% MCF-7 in 5x104 total cells. After 5 days of culture, two lines cross were traced on the bottom of the well. An inverted microscope with 10X objective was used to photograph at time 0 hours, 24 hours, 48 hours and 120 hours until confluence. We analyzed the phenotypic changes and mean migration time of cell culture and co-culture. The confluence of the cell layer of the area of migration occurred after 120 hours only in co-culture of MSC 30% and 70% MCF-7(163,66μµm to 0 µm). Flow cytometry: MSC cells were seeded into 6 well plates and co-cultured with MCF-7 cells in the same proportions as described above in 1x105 total cells, and maintained at 37° C with 5% CO2 for 5 days. Were quantified cells with CD44+/CD24- profile with anti-CD44 antibody (APC) and anti-CD24 (PE). The ANOVA with Tukey's post-test were used for statistical analysis. Data were presented as mean ± standard deviation, p< 0.05. There was no significant difference in CD44+/CD24- in the comparison groups: MSC vs. cell co-cultures; cell co-cultures vs. cell co-cultures; MCF-7 vs. 1% MSC+ 99% MCF-7; MCF-7 vs. 10% MSC + 90% MCF-7. We observed a significant increase in CD44+/CD24- population comparing MCF-7 vs. co-culture of 30% MSC + 70% MCF-7 (95% CI of diff, -55.56 to -27.71). Conclusions: The co-culture with MSCs an MCF-7 is associated with an increase in cell migration and, the is a increase in CD44+/CD24- cells. These findings suggest that the interaction of mesenchymal stem cells with MCF-7 may be is able to acquire potential metastatic profile. Citation Format: Fernanda Marques Rey, Willian Abraham da Silveira, Heriton Marcelo Ribeiro Antonio, Renata D Sicchieri, Daniel Guimaraes Tiezzi. Interaction of mesenchymal stem cell with breast cancer lineage cells and evaluation of their biological behavior [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P2-07-08.