Abstract P078: Cardiovascular Autonomic Impairment Triggers Cardiometabolic Dysfunction in an Experimental of Metabolic Syndrome

Abstract

The aim was evaluate on time-course of changes in cardiometabolic dysfunction model. Male Wistar and SHR rats were divided: Control (C), hypertensive (H), hypertensive + fructose (HF). The fructose overload (100g/L) was initiated at 30 days (d) of life. All evaluations were at 37, 45, 60 and 90 d of age. Arterial pressure (AP) signals were directly recorded. The baroreflex sensitivity was evaluated by the tachycardic (TR) and bradycardic (BR) responses to AP changes. Cardiovascular autonomic modulation was evaluated by spectral analysis. Inflammatory markers were evaluated in adipose tissue. No differences were observed in glycemia and triglycerides between the groups. The SHR groups showed increased systolic (SAP), diastolic (DAP) and mean arterial pressure (MAP) compared to control groups in all times of evaluation. Additionally, SHR groups progressively increased AP during the time course evaluation. The HF group showed additionally increased in DAP and MAP (90: MAP: C: 114±1, H: 165±3, HF: 179±3 mmHg) vs. H group. The cardiac parasympathetic modulation (HF-IP) was decreased H group in 37 d and HF group in 37, 90 d vs. C group. The VAR-PAS was higher in SHR groups vs. C group in all times evaluated. The HF group showed additionally increase in VAR-PAS during the time course evaluation (45, 90 vs. 37 d). The vascular sympathetic modulation (LF-PAS) was higher H group in 60, 90 d vs. C group. The HF group showed increased LF-PAS in 45, 60, 90 d vs. C group, and vs. 37 d in the same group; and showed additionally increased in 90 d vs. H group (90: C: 3.7±0.6 H: 6.2±0.9 HF: 8.7±0.5 mmHg2). The spontaneous baroreflex sensitivity was lower in SHR groups all the times evaluated (90: C: 0.80±0.1 H: 0.51±0.05 HF: 0.32±0.02 ms/mmHg). The H group showed decreased BR in 60, 90 d vs. C group, the HF group showed decreased in 37, 45, 60, 90 d vs. C group (90: C: 1.15±0.1 H: 0.80±0.05 HF: 0.77±0.07 bpm/mmHg). The HF group showed decreased TR in 60, 90 d vs. C group. Increased TNF alpha and IL-6 were observed only in 45 and 60 d in HF group. The results showed that cardiometabolic dysfunctions are time-dependent in SHR, and fructose overload induces additional dysfunctions in this model. Furthermore, the baroreflex impairment seems to precede hemodynamic, metabolic and inflammatory changes in this model.