Abstract P016: Circulation immune landscape in canonical pathogenesis of colorectal cancer by CyTOF analysis

Abstract

Abstract Objective: The present study aims to firstly describe the circulation immune landscape in the canonical pathogenesis of colorectal cancer (CRC) by detecting the peripheral white blood cell using mass cytometry by time-of-flight (CyTOF) technology. Methods: A total of 42 healthy controls (HC), 47 colorectal adenoma (CRA) patients, and 102 CRC patients were enrolled and their pathological information were also collected. A panel of 42 cell surface antigen markers were detected by mass spectrometry flow cytometry after peripheral blood mononuclear cells (PBMC) separated. The expression differences of various cell subsets among the three groups were further compared, and the cell subsets with different expressions were screened out for in-depth analysis. Results: We annotated the PBMC as T cells, B cells, NK cells and myeloid cells, and performed identification and component comparison of cell subgroups respectively. Compared with HC and CRA, NKT cell subsets were significantly reduced in CRC, while Naïve CD4+ T cells, effector CD8+ T cells and central memory CD8+ T cells were increased. Compared with HC, Treg cells increased in CRA, and central memory CD4+ T increased in CRC; but Naïve CD8+ T cells and Naïve double-negative T (DNT) cells decreased in both CRA and CRC. Compared with HC and CRA, Naïve B cells were significantly decreased in CRC; but switched memory B cells were increased. CD16- NK cells were significantly higher in CRC while CD16+ NK cells were decreased compared with HC. CD16- monocytes were increased, but CD16+ monocytes were decreased in CRC when compared to HC. Basophils and monocytes were significantly increased in CRC compared to CRA. Furthermore, we also found Effector CD4+ T cells and Naïve B cells increased during lymph node metastasis, while unswitched B cells, plasmablast and basophils decreased. Conclusion: We had successfully described the immune landscape of the canonical pathogenesis of CRC, and we further analyzed the changes in cell subsets related to the occurrence of lymphatic metastasis. Citation Format: Ke-Feng Ding, Xiang-Xing Kong, Jia-Sheng Xu, Yu-Rong Jiao, Ye-Ting Hu, Qian Xiao, Xu-Ran Hao, Zong-Bao Gao, Jun Li. Circulation immune landscape in canonical pathogenesis of colorectal cancer by CyTOF analysis [abstract]. In: Abstracts: AACR Virtual Special Conference: Tumor Immunology and Immunotherapy; 2021 Oct 5-6. Philadelphia (PA): AACR; Cancer Immunol Res 2022;10(1 Suppl):Abstract nr P016.