Abstract P-315: EFFECT OF NEOTYPE RECTAL MILD HYPOTHERMIA COOLING METHOD ON INTESTINAL BACTERIAL TRANSLOCATION IN HYPOXIC-ISCHEMIC BRAIN DAMAGE

Abstract

Aims & Objectives: Neotype rectal mild hypothermia cooling method was designed previously. The present study investigated the effect on bacterial translocation in rats with hypoxic-ischemic brain damage. Methods Design: A total of 60 male rats were randomly divided into group control, normothermia, cooling blanket, and rectal cooling. Plasma diamine oxidase (DAO) and D-lactate were measured separately at 0, 12, and 24h after HIBD. Spleen, liver, mesenteric lymph nodes (MLN) and rectum tissue were harvested and analyzed. Setting and Participants: Children’s hospital of Chongqing Medical University located in China. Interventions: Group CB was interfered by cooling blanket, and group RC interfered by rectal cooling. Results The integrity of the rectum tissue of rats in group RC was superior to that of CB and NT groups with less inflammatory infiltration under mucosa in the former group. Escherichia coli was found in the culture plate containing tissues of MLN in the RC group. On the other hand, Escherichia coli, Proteus vulgaris, Stenotrophomonas maltophilia, and Acinetobacter lwoffii were detected in culture plates containing tissues of MLN, spleen, and liver in NT and CB groups. The concentration of DAO and D-lactate decreased at 12h in group RC, which was predominant than that in group CB, and increased at 24h in both RC and CB groups (P<0.05). Conclusions Neotype rectal mild hypothermia cooling method could be a potential therapeutic strategy to maintain the integrity of rectal histology and intestinal microecology,reduce the possibility of inflammatory cell attack, intestinal mucosal permeability, and BT incidence.