Abstract LB-520: PTPN14 interacts with and negatively regulates the oncogenic function of YAP

Abstract

Abstract ABSTRACT The Hippo signaling pathway regulates cellular proliferation and survival, thus exerting profound effects on normal cell fate and tumorigenesis. The pivotal effector of this pathway is YAP, a transcriptional co-activator amplified in mouse and human cancers where it promotes epithelial-to-mesenchymal transition (EMT) and malignant transformation. Using IP Mass-spec analysis, we have identified a novel regulatory mechanism for the YAP oncogenic function via direct interaction with non-receptor tyrosine phosphatase 14 (PTPN14). PTPN14 interacts with YAP through PPxY domain, and overexpression of PTPN14 increases the inactive cytoplasmic form of YAP and decreases the transcriptional co-activator activity of YAP. Moreover, knockdown of PTPN14 induces nuclear retention of YAP and increases YAP-dependent cell migration. In summary, our study indicate a potential tumor-suppressor role for PTPN14 in the Hippo pathway and demonstrate a novel mechanism in YAP regulation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-520. doi:1538-7445.AM2012-LB-520