Abstract LB-52: Increased incidence of cancer in the transgenic mice with 8-Lipoxygenase knocked out.

Abstract

Abstract 15-Lipoxygenase-2 (ALOX15B), an oxidoreductase in the metabolism of arachidonic acid, is a functional tumor suppressor whose expression is reduced in a variety of human cancers. To determine the roles of ALOX15B in carcinogenesis, we generated transgenic mice with ALOX8, the murine homolog of ALOX15B, knocked out. ALOX8 expression at mRNA level was abolished in homozygous knockout mice and reduced to half of wild type in heterozygous knockout mice. It's observed that homozygous female ALOX8 knockout mice are infertile but male ALOX8 knockout mice are fertile. Increased incidence of tumor as uni or multimass was found in the lung, prostate and in the mesentery region of homozygous and heterozygous ALOX8 knockout mice, when compared with little mate wild type mice. Histological evaluations showed an increase in secondary tumors and inflammation in different tissues like lung and large intestine in mice with ALOX8 knocked out. The transgenic mice could be a good model to study the role of 8-lipoxygenase in carcinogenesis and may provide insights into the tumor suppressing activities of human ALOX15B. Citation Format: Rana Al-Fardan, Rabindra Karki, Changxiong Guo, Daotai Nie. Increased incidence of cancer in the transgenic mice with 8-Lipoxygenase knocked out. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr LB-52. doi:10.1158/1538-7445.AM2013-LB-52