Abstract LB-124: Preliminary anti-proliferative effect of ethanolic extracts of Xylopia aethiopica on prostate and breast cancer cell lines

Abstract

Abstract Xylopia aethiopica (African Pepper), a plant found throughout West Africa, has both nutritional and medicinal uses. The aim of this research was to study the preliminary anti-proliferative effects of ethanolic extracts of Xylopia aethiopica on prostate and breast cancer cell lines. Dried X. aethiopica fruits were extracted with 70% ethanol and tested against prostate(LNCaP) and breast cancer (MCF7 and MDA-MB231) cells’ viability in vitro using the MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide) assay. The IC50 values for the extract were determined through linear regression using GraphPad Prism 6 software. The data obtained were expressed as mean ± SEM. Statistical analysis was carried out by one-way ANOVA followed by Dunnet's test (α = 0.05) to compare experimental means with controls. The data generated indicates that X. aethiopica fruits extract showed a dose-dependent anti-proliferative activity against LNCaP, MDA-MB231and MCF7 cancer cell lines after treatment for 48 and 72 hours. The extract induced a 16.96% and 93.5% inhibition on MDA-MB231 cells, 29.76% and 94.03% on the MCF7 cells and a 9.15% and 94.61% inhibition on the LNCaP cells at the lowest (1μg/ml) and highest (100μg/ml) dose respectively after 48 hours. The extract induced a 12.26% and 91.8% cell growth inhibition on the MDA-MB231 cells, 3.35% and 87.36% growth inhibition on the MCF7 cells and 2.28% and 92.42% cell growth inhibition on the LNCaP cells at the lowest (1μg/ml) and highest (100μg/ml) dose respectively after 72 hours. The IC50 was estimated to be 3.408μg/ml, 2.064μg/ml, 3.371μg/ml for MDA-MB231, MCF7 and LNCaP cells respectively. The data generated from this research indicates the anti-proliferative effects of ethanolic extract of X. aethiopica fruits on breast and prostate cancer cells. This can be seen from the inhibition of cell growth by this extract thus highlighting its potential as a therapy against breast and prostate cancer. Citation Format: Emeka E.J Iweala, Eunice W. Bankole. Preliminary anti-proliferative effect of ethanolic extracts of Xylopia aethiopica on prostate and breast cancer cell lines. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr LB-124.