Abstract LB-116: sLZIP induces migration and invasion of cervical cancer cells through expression of matrix metalloproteinase-9

Abstract

Abstract Extracellular proteolysis mediates tissue homeostasis. In cancer, altered proteolysis leads to abnormal tumor growth, inflammation, tissue invasion, and metastasis. Chronic infections and inflammation are major risk for various types of cancer. The matrix metalloproteinases (MMPs) represent the most prominent family of proteinases associated with inflammation and tumorigenesis. Matrix metalloproteinase-9 (MMP-9) is involved in a wide range of normal and pathologic conditions, including inflammation, tissue repair, tumor invasion, and metastasis. sLZIP is a member of the zinc-finger transcription factor family. However, role of sLZIP in MMPs expression is previously unknown. Our results showed that sLZIP increased the mRNA level and the proteolytic activity of MMP-9 in HeLa cells. sLZIP also increased the transcription activity of MMP-9 by binding to the CRE of the MMP-9 promoter region. The requirement for sLZIP in MMP-9 expression was further supported by the fact that HeLa cells expressing sLZIP siRNA was refractory to MMP-9 expression. Results from wound healing and invasion assays showed that sLZIP enhanced migration and invasion of cervical cancer cells. Increased migration and invasion of HeLa cells induced by sLZIP was restored by inhibition of the proteolytic activity of MMP-9. Our results indicate that sLZIP induces expression of MMP-9 and is probably involved in invasion and metastasis of cervical cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-116. doi:10.1158/1538-7445.AM2011-LB-116