Abstract LB-462: Chemopreventive effects of freeze-dried strawberries in inflammation-associated mouse colon tumorigenesis

Abstract

Abstract Colorectal cancer (CRC) is one of the most common malignant neoplasms in the United States. The risk for CRC is associated with extent and duration of inflammatory bowel disease (IBD). Patients with IBD are at increased risk of developing CRC when compared to the general population. Therefore, primary cancer chemoprevention is an important strategy to reduce the risk of CRC in IBD patients and thus, this approach has recently received more attention. Our laboratory has conducted extensive research on cancer chemoprevention in GI tract. In our previous study, we found that dietary freeze-dried black raspberries significantly inhibited adenoma and adenocarcinoma formation and decreased the level of oxidative stress in an azoxymethane (AOM)-induced rat CRC model. In a carcinogen-induced rat esophageal cancer model, we found that dietary freeze-dried strawberries significantly inhibited tumor development. However, the effects of strawberries in inflammation-associated CRC have not been investigated as yet. The principal objective of this study is to investigate the inhibitory effects of dietary freeze-dried strawberries in AOM/Dextran Sodium Sulfate (DSS)-induced mouse colon carcinogenesis. At 4–5 weeks of age, male Crj: CD-1 mice were given a single intraperitoneal injection of AOM (10mg/kg body weight), followed by 2% DSS in their drinking water for seven days, starting one week after the AOM injection. After 3 weeks, animals were fed AIN-76A diet or AIN-76A diet containing 10% freeze-dried strawberries. At week 20, mice were sacrificed for histopathological examination. We found that strawberries significantly decreased tumor incidence from 100% in mice treated with AOM/DSS only to 44.4% in mice treated with AOM/DSS plus strawberries (55.6% reduction, P = 0.02). We also found that the average animal body weight was significantly higher (P = 0.036) in animals fed strawberries (41.9 ± 5.3 g) when compared to animals fed control diet (38.7 ± 3.7 g). A large-scale investigation of the inhibitory effects of strawberries on biomarkers associated with colon carcinogenesis is under way. (Supported by California Strawberry Commissions) Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-462. doi:10.1158/1538-7445.AM2011-LB-462