Abstract LB-276: 2-D DIGE and siRNA to find new cancer targets

Abstract

Abstract siRNA is a powerful tool in loss-of-function studies which generates valuable information on target validation. siRNA is highly selective and suppress gene expression in a sequence-specific manner. These features have made it a key tool in signal transduction - a cornerstone in cancer biology. 2-D DIGE (two dimensional differential gel electrophoresis) is a fluorescence based technology with the capacity to separate thousands of proteins in one single run, with high reproducibility. This makes it a valuable tool for differential expression analyses. In this study two powerful methods, siRNA and 2-D DIGE, were combined to investigate a well-known signaling pathway, pivotal in cancer biology. Silencing of the target protein made it possible not only to identify a large number of proteins that were differentially regulated but also to resolve the time dependency of protein regulation. Thus the combined use of siRNA and 2-D DIGE made it possible to find potentially new targets in the signaling pathway. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-276. doi:1538-7445.AM2012-LB-276