Abstract
Abstract The ability to sequence genomes has far outstripped approaches for deciphering the information they encode. We have developed a suite of techniques based on ribosome profiling (deep sequencing of ribosome protected fragments) that dramatically expand our ability to follow translation in vivo. I will present recent applications of our ribosome profiling approach including the following: (1) Development of ribosome profiling protocols for a wide variety of eukaryotic and prokaryotic organisms. (2) Uses of ribosome profiling to globally monitor when chaperones, targeting factors or processing enzymes engage nascent chains. (3) Application of ribosome profiling to define the protein coding potential of complex genomes. (4) The development of a proximity-specific ribosome profiling assay for monitoring subcellular localized translation and its use to follow translation on the surface of the endoplasmic reticulum and mitochondria. Citation Format: Jonathan Weissman. Monitoring translation in space and time with ribosome profiling. [abstract]. In: Proceedings of the AACR Special Conference on Translational Control of Cancer: A New Frontier in Cancer Biology and Therapy; 2016 Oct 27-30; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2017;77(6 Suppl):Abstract nr IA20.
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