Abstract IA05: Genome-wide views into the mechanisms of transcription regulation

Abstract

Abstract RNA Polymerase II (Pol II) is the molecular machine that transcribes all the protein-encoding mRNAs as well as a spectrum of non-coding regulatory RNAs. The distribution of Pol II reveals not only location and levels of transcription of these transcribed genes, but also, the distinctive patterns of divergent transcription that can be used to identify the active promoters and enhancers that regulate these genes. Moreover, by monitoring changes in the distribution of Pol II across genomes in response either to regulatory signals and to directed perturbations of transcription factors, the distinct steps in transcription that are regulated can be elucidated. With increasing positional and temporal resolution provided by new, genome-wide nuclear run-on and chromatin assays, we are able to gain critical insights into the mechanisms governing how polymerases initiate and elongate through transcription units, and into how these processes are regulated. In this presentation, I will provide a comprehensive genome-wide view of the rapid and dramatic changes in transcription that accompany the stress response, a long used model for studying regulation, and the factors and molecular mechanisms that regulate this reprogramed transcriptional response in both Drosophila and mammals. Citation Format: John T. Lis, Charles Danko, Fabiana Duarte, Dig Bijay Mahat. Genome-wide views into the mechanisms of transcription regulation. [abstract]. In: Proceedings of the AACR Special Conference on Chromatin and Epigenetics in Cancer; Sep 24-27, 2015; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2016;76(2 Suppl):Abstract nr IA05.