Abstract IA02: Genomic organization of yeast chromatin and gene regulatory complexes

Abstract

Abstract The large number of genes across eukaryotic genomes begs the question as to whether they are all fundamentally regulated in the same manner. Clearly, sequence-specific factors direct gene-specific regulation, but then do all downstream events proceed along a common path? To begin to address this question, we use ChIP-exo to map the precise contact points of proteins along genomic DNA. The near single-bp readout provides structural insights into how proteins are organized into complexes across genomes, and how they might position nucleosomes to regulate gene expression. Multiple classes of complexes occupy nucleosome-free promoter regions: an ensemble of sequence-specific factors and their coactivators, the core transcription machinery, chromatin remodelers and nucleosomes. This talk will focus on identifying common principles that determined how factors and chromatin organize themselves at promoters across co-regulated genes, and in genes in general. Citation Format: B. Franklin Pugh. Genomic organization of yeast chromatin and gene regulatory complexes. [abstract]. In: Proceedings of the AACR Special Conference on Chromatin and Epigenetics in Cancer; Sep 24-27, 2015; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2016;76(2 Suppl):Abstract nr IA02.