Abstract C28: Ligand-conjugated Biosynthesized Superparamagnetic Iron Oxide Nanoparticles for the Detection of Triple Negative Breast Cancer

Abstract

Abstract This paper presents experimental results of the in vitro and in vivo studies combined with immunofluorescence staining that demonstrate the detection of triple negative breast cancer using conjugated biosynthesized superparamagnetic iron oxide nanoparticle (BSPIONs). Insights on nanoparticles-cells interactions at a nanoscale that are guided by specificity and selectivity in the targeting of triple negative breast cancer cells (MDA MB 231 cells) are being explored using atomic force Microscope (AFM). This study also focuses on the use of ligand-conjugated BSPIONs as targeted contrast agents in magnetic resonance imaging (MRI) at an in vitro and in vivo level. The ligands conjugated to BSPIONs are shown (revealed by confocal images via immunofluorescence staining) to have receptors that are overexpressed on the surfaces of triple negative breast cancer cells. The results suggest that conjugated BSPIONs can be used for rapid screening of potentials ligands for specific targeting of receptors that are over-expressed on the surfaces of breast cancer cells. The implication of this work are discussed for the design of robust ligand-conjugated BSPIONs with the appropriate nanoparticles-cells interaction as a contrast agent for enhancing MRI for the detection of triple negative breast cancer. Citation Format: John David Obayemi, Jingjie Hu, Vanessa O. Uzonwanne, Olushola S. Odusanya, Karen Malatesta, Nicolas Anuku, Winston O. Soboyejo. Ligand-conjugated Biosynthesized Superparamagnetic Iron Oxide Nanoparticles for the Detection of Triple Negative Breast Cancer. [abstract]. In: Proceedings of the Ninth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2016 Sep 25-28; Fort Lauderdale, FL. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2017;26(2 Suppl):Abstract nr C28.