Abstract B4: MicroRNAs differentially expressed in preoperative human serum of ovarian cancer

Abstract

Abstract MicroRNAs (miRNAs) have been reported to play important regulatory roles in many biological processes and tumorigenesis. To investigate the roles of serum miRNAs in patients before tumor bulk was surgically removed from ovarian cancer patients, we utilized miRNA real-time QPCR profiling technology to characterize the miRNAs in pre-operative and postoperative sera. Six pairs of sera were analyzed using 270 miRNA SyberGreen probes. Upregulation of miR187, miR499-3p, miR548c-5p, miR1, miR107, miR125a-5p, miR18b were identified in the preoperative sera. Targetscan was utilized to identify Dab2 as the target gene of miR187. In vitro studies of SKOV3 cells showed that ectopic expression of miR187 suppressed Dab2 and resulted in increased cell proliferation. We concluded that miR187 may be a potential biomarker in sera of ovarian cancer patients. Further studies with larger sample sizes are warranted to validate our results. Citation Format: Angel Chao, Tzu Hao Wang, Hua-Chien Chen, Shu-Jen Chen, Chyong-Huey Lai. MicroRNAs differentially expressed in preoperative human serum of ovarian cancer [abstract]. In: Proceedings of the AACR Special Conference on Noncoding RNAs and Cancer; 2012 Jan 8-11; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(2 Suppl):Abstract nr B4.