Abstract
Abstract Dysregulated expression of microRNAs (miRNAs) is confirmed to be closely associated with tumor development, progression, and carcinogenesis. The purpose of the present study was to determine the miRNA expression profile of gastric cancer and to explore the relationship between aberrant miRNA expression and prognosis of gastric cancer in Chinese patients. Among a total of 1,146 miRNAs analyzed, 16 miRNAs were found to be present at significantly different expression levels in tissues from gastric cancer compared to those matched adjacent non-tumor tissues (ANTTs). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was then employed to characterize the expression patterns of miR-193b and miR-196a in total 96 snap frozen clinical gastric samples including 48 gastric cancer tissues and 48 paired ANTTs. The association between miRNA expression and clinical-pathological characteristics of patients was analyzed by binary logistic regression. We found that down-expression of miR-193b was significantly correlated with Lauren type, differentiation, UICC stage, invasion, and metastasis of gastric cancer (P < 0.05), while up-expression of miR-196a was significantly correlated with poor differentiation (P = 0.022). Moreover, down-expression of miR-193b elevated cancer risk in UICC stage (adjusted odds ratio = 8.69; 95% confidence interval = 1.06-56.91; P = 0.043;). A Kaplan-Meier survival curve indicated that the patients with high fold change of down-regulated miR-193b had a significantly shorter survival time (n = 19; median survival = 29 months) compared to patients with low fold change of down-regulated miR-193b (n = 29; median survival = 54 months) (P = 0.001). Overall survival time of patients with low fold change of up-regulated miR-196a (n = 27; median survival = 52 months) was longer than that of patients with high fold change (n = 21; median survival = 46 months) (P = 0.003). Thus, miR-193b and miR-196a may be applied as novel and promising prognostic factors in gastric cancer. Note: This abstract was not presented at the conference. Citation Format: Wenjie Sun, Yongping Mu, Xiulan Su. Association of down-regulation of miR-193b and up-regulation of miR-196a with prognosis in gastric cancer. [abstract]. In: Proceedings of the Thirteenth Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2014 Sep 27-Oct 1; New Orleans, LA. Philadelphia (PA): AACR; Can Prev Res 2015;8(10 Suppl): Abstract nr B25.
Published Version
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