Abstract B22: The synergistic effect of a sulforaphane and maitake mushroom extract combination on the expression of the detoxifying enzymes NQO1 and UGT1A1

Abstract

Abstract Excess estrogen exposure, whether from endogenous estrogen, harmful estrogen metabolites, or exogenous xenoestrogens (e.g. BPA, parabenes, etc.) can be detrimental to overall breast health. Here we describe a novel blend of natural ingredients including sulforaphane and maitake-derived glucans and their effect on detoxifying enzymes capable of diminishing excess estrogens. Sulforaphane, an isothiocyanate found in broccoli seeds and sprouts, results from the hydrolization of a glucosinolate called glucoraphanin by the enzyme myrosinase. In vitro and in vivo studies reveal the ability of sulforaphane to induce Phase 2 detoxifying enzymes (e.g. NAD(P)H:Quinone Oxidoreductase (NQO1) which functions to rid the body of harmful quinone estrogen metabolites and UDP-Glucuronosyltransferase 1-1 (UGT1A1) which has been shown to play a role in the metabolism of Bisphenol-A, an estrogen mimic, via glucoronidation). Previous research involving maitake extracts has largely focused on their immune-boosting properties. We examined the effect of the combination of sulforaphane and maitake-derived glucans on NQO1 and UGT1A1. RAW 246.7 mouse monocyte/macrophages were treated with sulforaphane and maitake mushroom extract alone or in combination. mRNA levels of NQO1 and UGT1A1 were assessed via quantitative RT-PCR revealing a synergistic effect on the induction of both NQO1 and UGT1A1 gene expression. Further, we carried out an in vivo study in rats during which the animals were dosed with sulforaphane, maitake mushroom extract, or the combination for 14 days. The upregulation of UGT1A1 gene expression was greater in the mammary glands of the rats given the combination of sulforaphane and maitake extract compared to those receiving either agent alone. This study demonstrates the synergistic effect of the combination of sulforaphane and a maitake extract on the gene expression of Phase 2 detoxifying enzymes NQO1 and UGT1A1 suggesting the agents together may be effective at detoxifying the body of excess estrogens and promoting breast health. Citation Format: Grace A. Cornblatt, Stacy Ownby, Brian S. Cornblatt. The synergistic effect of a sulforaphane and maitake mushroom extract combination on the expression of the detoxifying enzymes NQO1 and UGT1A1. [abstract]. In: Proceedings of the Twelfth Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2013 Oct 27-30; National Harbor, MD. Philadelphia (PA): AACR; Can Prev Res 2013;6(11 Suppl): Abstract nr B22.