Abstract B174: KIOM 210102 inhibits proliferation of colon cancer cells by G2/M phase arrest.

Abstract

Abstract KIOM210102 is ethanol extract, a medicinal plant belonging to the Aristolochiaceae that has been used to cure for pain and fever traditionally in Korea, Chinese and Japan. It has also been used for treatment of inflammation, allergy and histamine-related disease. Our preliminary study showed that KIOM210102 has a potent cytotoxic effects on several cancer cell lines including HCT116, a human colon carcinoma cell. Here we attempted to assess the anti-carcinogenic mechanism of KIOM210102 in human colon carcinoma cell line. HCT116 cells were treated with different concentrations of KIOM210102 for 48 hrs for cell viability measured by MTS assay. At a concentration of 15.8×0.68 ug/ml, KIOM 210102 inhibited 50% of proliferation of HCT116. The cell cycle distribution and apoptosis were measured by flow cytometry using Propidium Iodide staining and immunoblotting method was used to study parameters associated with apoptosis and cell proliferation. Our data revealed that there was an acute G2/M cell cycle arrest followed by massive cell death in the cells treated with KIOM210102. The effect of KIOM210102 was mediated by increased p53 and p21waf/cip expression and decreased cyclin B. These processes were accompanied by caspase-3 activation and PARP cleavage and down regulation of the anti-apoptotic protein Bcl-2. These results suggest that KIOM210102 has a potential for a novel herbal anti-cancer therapeutics. This research was supported by a grant from the Korea Institute of Oriental Medicine (KIOM, K11061). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2011 Nov 12-16; San Francisco, CA. Philadelphia (PA): AACR; Mol Cancer Ther 2011;10(11 Suppl):Abstract nr B174.