Abstract B14: Tanshinones suppress AURKA through up-regulation of miR-32 expression in non-small cell lung cancer

Abstract

Abstract Tanshinones which includes tanshinone I (T1), tanshinone IIA (T2A), and cryptotanshinone (CT), are the liposoluble constituent of Salia miltiorrhiza, AURKA, an oncogene, encodes a serine-threonine kinase which regulates mitotic processes in mammalian cells. Here, we reported that tanshinones mediate AURKA suppression partly through up-regulating the expression of miR-32, one of miR-25 family. We found that tanshinones could inhibit cell proliferation, promote apoptosis, and impede cell-cycle progression, thus performing an antineoplastic function in non-small cell lung cancer (NSCLC). Additionally, we demonstrated that tanshinones attained these effects in part by down-regulating AURKA, corroborating previous reports. Our results showed that in NSCLC, similar effects were obtained with knock-down of the AURKA gene by siRNA. We also verified that AURKA was the direct target of miR-32. we also found that miR-32 can promote the expression of Let-7 family, however, let-7 family down-regulates the level of miR-32. Collectively, our results demonstrated that tanshinones could inhibit NSCLC by suppressing AURKA via up-regulating the expressions of miR-25 family, let-7 family and other related miRNAs. Note: This abstract was not presented at the conference. Citation Format: zhongliang ma, bingjie zhang, yanli li. Tanshinones suppress AURKA through up-regulation of miR-32 expression in non-small cell lung cancer. [abstract]. In: Proceedings of the AACR Special Conference on Noncoding RNAs and Cancer: Mechanisms to Medicines ; 2015 Dec 4-7; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2016;76(6 Suppl):Abstract nr B14.