Abstract A76: Comparative mRNA and microRNA expression profiling of canine mammary carcinoma cell lines and macrophages grown as a co-culture in vitro.

Abstract

Abstract Solid tumors comprise of various cells, including cancer cells, resident stromal cells, migratory haemopoietic cells and other. These cells regulate tumor growth and metastasis. Macrophages constitute probably the most important element of all interactions within the tumor microenvironment. However, the molecular mechanism, that guides tumor environment, still remains unknown. Exploring the underlying molecular mechanisms that orchestrate these phenomena has been the aim of our study. The co-cultures of five various canine mammary carcinoma cell lines and macrophages were established and maintained for 72 hrs. After that, having sorted the cells, an integrated analysis of genome-wide mRNA and microRNA expression profiles have been assessed. The analysis showed that the up-regulated genes in the cancer cell lines grown as co-culture with macrophages are involved mainly in: macrophages activation, cell motion, mammary gland development, cell-cell adhesion, and angiogenesis. Basides, the presence of macrophages in the cancer environment induces acquisition of the macrophage antigens and phenotype in cancer cells. The up-regulated genes in macrophages grown as co-cultre with cancer cells were involved mainly in cell-cell adhesion and cell-matrix adhesion. We found that co-culture of cancer cells and macrophages regulates expression of several important miRNAs. Taken together, this integrated comparative study generated a scheme of the molecular changes that occur during interactions between macrophage and cancer. Because as far as we realize this is the first analysis of changes in miRNA expression in cancer cells due to a presence of macrophages, our study can serve as a valuable source for future studies on a tumor microenvironment, and some of the highlighted genes, miRNAs, pathways or processes may be useful for diagnostic or therapeutic purposes. This work was supported by grant no N N308012939 from the Ministry of Sciences and Higher Education. Citation Format: Magdalena Król, Karol Pawlowski, Kinga Majchrzak, Alicja Majewska, Tomasz Motyl. Comparative mRNA and microRNA expression profiling of canine mammary carcinoma cell lines and macrophages grown as a co-culture in vitro. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology: Multidisciplinary Science Driving Basic and Clinical Advances; Dec 2-5, 2012; Miami, FL. Philadelphia (PA): AACR; Cancer Res 2013;73(1 Suppl):Abstract nr A76.