Abstract A65: Proteomics of normal, pancreatitis and pancreatic cancer human tissues

Abstract

Abstract Pancreatic cancer is one of the most aggressive malignancy with an increased in incidence predicted, particularly in African Americans. Pancreatic cancer is considered a silent disease with poor prognosis and a lack of early biomarkers for detection. Proteomics has been applied in a number of diseases for identifying or discovering biomarkers. It has long been suggested that chronic pancreatitis may put some individuals at risk for developing pancreatic cancer. This study identified proteins that are increased in pancreatic cancer samples using proteomic technology. Proteins were extracted from tissues and separated in 2-D PAGE and imaged. PDQuest 2D image analysis software (Bio-Rad, USA) was used for the automated detection of spots. Representative proteins, overexpressed in tumor and not in the normal tissues, were excised from gels, subjected to in-gel digestion, and analyzed by MALDI-TOF mass spectrometry. Proteins identified included transferrin (Access number (AN) 4557871), ER-60 protein (AN 2245365), proapolipoprotein (AN 178775), tropomyosin 1 (AN 27597085), alpha 1 actin precursor (AN 4501881), ACTB protein (AN 15277503), gamma 2 propeptide (AN 4501889) and an unnamed protein product (AN 921757045). Albumin was abundantly expressed in pancreatic cancer compared to normal samples. Several proteins, which were shown in pancreatic cancer, were also observed in pancreatitis samples. Understanding the role of these specific proteins and their mechanistic action will give insights into their involvement in pancreatic cancer. Citation Format: Sulma Mohammed, George Hammons, Beverly D. Lyn-Cook. Proteomics of normal, pancreatitis and pancreatic cancer human tissues. [abstract]. In: Proceedings of the Seventh AACR Conference on The Science of Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; Nov 9-12, 2014; San Antonio, TX. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2015;24(10 Suppl):Abstract nr A65.