Abstract A46: Exposure of quercetin, kaempferol and exogenous glutathione on the expression levels of phospho- and total-Akt in 3T3-L1 preadipocytes

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Abstract Obesity has been reported to be a risk factor for several types of cancer. The Akt pathway or PI3K-Akt pathway is a signal transduction pathway that promotes survival and growth in response to extracellular signals. Key proteins involved are phosphatidylinositol 3-kinase (PI3K) and Akt, or Protein Kinase B. Activated Akt mediates downstream responses, including cell survival, growth, proliferation, cell migration and angiogenesis, by phosphorylating a range of intracellular proteins. The effects of the exposure of several doses each of the flavonoids quercetin and kaempferol and that of exogenous glutathione (GSH) (at 0, 5 10, 15, 20 and 25 μM doses) for 24 hr on the expression levels of phospho- and total-Akt levels in 3T3-L1 preadipocytes were investigated. Phospho-, total-Akt and the expression levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH, which was used as an internal standard) were assayed by the MILLIPLEX® MAP mates protocol, based on the Luminex® xMAP® technology (Millipore Corp. St. Charles, MI). There were no significant differences in the expression levels of GAPDH for the tested compounds and at the different concentrations. Significant (p<0.05) decreases however, in the espression levels of phospho-Akt in cells for the quercetin, kaempferol and GSH treated samples were observed. Total-Akt levels remained the same and, were not significantly different from each other for the tested compounds and at the various concentrations. The results may indicate that exposure of 3T3-L1 preadipocytes to quercetin, kaempferol and GSH did not result in the activation of Akt suggesting the role of such compounds in blocking cell differentiation in 3T3-L1 cells. Thus, quercetin, kaempferol and GSH may significantly cause a decrease in the proliferation of 3T3-L1 preadipocytes, suggesting an association with the low phosphorylation of the Akt protein. Citation Format: William Y. Boadi. Exposure of quercetin, kaempferol and exogenous glutathione on the expression levels of phospho- and total-Akt in 3T3-L1 preadipocytes. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2015 Nov 5-9; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2015;14(12 Suppl 2):Abstract nr A46.