Abstract A27: Proteomic study of the of posttranslational modifications (phosphorylation and glycosylation) and its significance in human breast cancer.

Abstract

Abstract Post translational modifications are the biochemical modifications introduced into the protein after translation (or during translation in case of co-translational modification). Various changes that occur are in the form of chemical groups added to various residues of the target protein by specific enzymes like glycosyltransferase, kinase, methyltransferase, etc. Various studies have shown that cancer cells differ from non-neoplastic cells not only in expression of proteins and their related function, but they also have major differences in the pattern of post-translational modification. These changes may be direct result of difference in expression levels of PTM-responsible enzymes, or differences in the functioning of the same. In the same manner, differences in PTM pattern of cancer proteins leads to many structural and functional changes in the target proteins, many of which may be responsible in furthering the neoplastic characteristics and survival of the cancer cell. Since PTM leads to change in functionality in target proteins, this study aims to find a direct relation between change in function of both pro-apoptotic and anti-apoptotic proteins and cancer cell immortality. Theoretically, pro-apoptotic proteins need to be non-functional for the cell to skip apoptosis, as is often seen in cancer cells. Also, the activity of anti-apoptotic proteins needs to be subdued by PTM to reach the same results. Differential PTM of various proteins in cancer cells with respect to non-neoplastic cells has been seen to be of great significance. This upregulation or downregulation of certain type of specific PTM can be a factor for the overall progression and development of cancer. Present study was desined with an aim to identifying the proteome exclusive to breast cancer cells, and also to study the differential PTM pattern of the same and determine the significance of this difference in various aspects of cancer occurrence, pathogenesis and progression. We found that the there is a significant change in the expression profiling of phosphorylation and glycosylation patterns of various proteins associated with breast cancer as assessed by using various bioinformatic based tools. Citation Information: Mol Cancer Ther 2013;12(11 Suppl):A27. Citation Format: Navkiran Kaur, Parul Jauhari. Proteomic study of the of posttranslational modifications (phosphorylation and glycosylation) and its significance in human breast cancer. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2013 Oct 19-23; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2013;12(11 Suppl):Abstract nr A27.