Abstract
Abstract Introduction: sphingosine 1-phosphate (S1P) is a bioactive lipid molecule that involves in tumor progressions, metastasis and chemo-resistance. We investigated the effects of S1P and its signaling mechanism in induction of tumor progression in chronic lymphoblastic leukemia SKW3 cells and non-small cell lung cancer H1299 cells. Methods: We investigated the effects of S1P on proliferation with MTT assay, invasion by soft-agar colony forming assay and cell migration by applying trans-well migration assay. The expression of survivin gene was assessed by Real-time PCR. Results: We showed that low concentrations of S1P induced proliferation and migration in both cell lines. S1P also stimulated invasiveness in H1299 cancer cells. We showed that the effects of S1P in tumor progression are S1P receptor-dependent. Survivin also plays a key role in S1P tumorogenic functions. Conclusion: Our results suggest that identifying cancer patients with higher levels of S1P and survivin and inhibiting their activities can be considered as an important strategy to increase the efficacy of chemotherapeutic agents. Note: This abstract was not presented at the conference. Citation Format: Nasser Samadi, Maryam Tabasi Nezhad, Parisa Ghanbari, Mahsa Mohseni. Sphingosine 1-phosphate promotes tumor progression in a survivin-dependent manner. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Drug Sensitivity and Resistance: Improving Cancer Therapy; Jun 18-21, 2014; Orlando, FL. Philadelphia (PA): AACR; Clin Cancer Res 2015;21(4 Suppl): Abstract nr A20.
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