Abstract
Abstract Apart from the man, the dog develops prostate cancer spontaneously. Although the diseases in both species have several clinical aspects in common, not much is known about the expression of markers in the canine prostate cancer due to the lack of study in canine prostate cancer research. Therefore, the vast majority of canine prostate cancers at the time of diagnosis are end-staged. Numerous prostate cancer cell lines have been developed, but few cell lines develop bone metastases and no cell lines have been reported to cause brain metastases except the Ace-1 and the Leo canine prostate cancer cell lines. Prostate-specific membrane antigen (PSMA), which is known as a prostate cancer biomarker in humans, its usefulness in canine specimens has not been examined fully in these cell lines. Although there has been a considerable effort toward the development of PSMA-targeted diagnostic and therapeutic technologies for dogs, methods for detecting circulating and invasive prostate tumor cells currently remain limited. Previously, we developed a prostate circulating tumor cell (PCTC) detection method by a fluorescent PSMA inhibitor. The focus of the study is to evaluate PSMA expression on the Ace-1 and Leo cell lines by fluorescent conjugated PSMA inhibitor in flow cytometry. It is expected that the results will enable the development of detecting PSMA expressing canine cells in circulation for prognosis and diagnosis of canine prostate cancer. Citation Format: Lisa Y. Wu, Tiancheng Liu, Bernard Vanwie, William C. Davis, Clifford E. Berkman. Molecular Detection of Prostate-Specific Membrane Antigen in Canine Prostate Carcinoma Cells [abstract]. In: Proceedings of the AACR Special Conference on Chemical Systems Biology: Assembling and Interrogating Computational Models of the Cancer Cell by Chemical Perturbations; 2012 Jun 27-30; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2012;72(13 Suppl):Abstract nr A20.
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