Abstract A197: Cancer associated fibroblasts are not form by epithelial-to-mesenchymal transition in nu/nu mice

Abstract

Abstract Cancer-associated fibroblasts are very important players to form specific cancer microenvironmnet and bioactive elements influencing the biological properties of malignant tumors. Their origin from different cell types has been established. It is suppose and under debate that exist three ways of the possibility of their formation: a) by epithelial-to-mesenchymal transition from cancer cells b) by activation of local mesenchymal cells c) by MSC (mesenchymal stem cell), which migrate from bone marrow to tumor. We focused on epithelial-to-mesenchymal transition. This study shows that human cancer cells lines FaDu FaDu (human squamous cell carcinoma isolated from pharynx; HTB-43), Sw620 (human colorectal adenocarcinoma; CCL-227) and HT-29 (human colorectal adenocarcinoma; HTB-38) grafted to nu/nu CD-1 mice induced formation of tumor stroma with the presence of typical smooth muscle actin-containing cancer-associated fibroblasts. These cells seem to be of the host origin because they are not recognized by an antibody specific for human vimentin, as was also verified in vitro. These results suggest that cancer-associated stromal fibroblasts are not formed by epithelial-to-mesenchymal transition from cancer cells. Citation Format: Pavol Szabo, Karel Smetana, Barbora Dvorankova, Rosana Mateu, Blanka Rihova. Cancer associated fibroblasts are not form by epithelial-to-mesenchymal transition in nu/nu mice. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2015 Nov 5-9; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2015;14(12 Suppl 2):Abstract nr A197.